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AG Ernährung

Main topics of the Research Laboratory of the Children's Hospital

  • Utilisation of 13C-labelled glycosyl ureides for oro-caecal transit time measurements
  • Fate of ingested doubly labelled probiotica
  • Impact of L-carnitine on fat oxidation
  • Effect of alcohol on liver detoxication capacity
  • Gastric emptying
  • Effect of resistant starch on colon metabolism and fat oxidation

PDF Lecture : "Stable isotopes in gastroenterology and nutrition"

International scientific activities

Co-operation partners:

Prof. Dr. Jörg Dötsch
University Hospital Cologne Department of Pediatrics

Dr. Frans Stellaard
University Medical Center Groningen Department of Laboratory Medicine

Dr. Frank Walther
University of Rostock Children's Hospital

Dr. Niels Rochow
McMaster Children's Hospital, Hamilton Canada

BASIS-member (Benelux Association of Stable Isotope Scientists)

Member of the GASIR (German Association for Stable Isotope Research)

Member of the Editorial Board of Isotopes in Environmental and Health Studies

Research awards

ESPEN Abbott Grant, Helsinki 1989
ESPEN Abbott Grant, Athens 1990
Milupa Preis, Frankfurt 1993

 

Publications 2004-2016

Book chapters, proceedings and monographs

  1. Heine W, Mohr C, Wutzke KD: Glykosylureide, Abschlussbericht. DFG-Projekt: He 2058/2-1/2-2, 1997.

Peer reviewed papers

  1. Wutzke KD, Glasenapp B: The use of 13C-labelled glycosyl ureides for evaluation of oro-caecal transit time. Eur J Clin Nutr 2004;58:568-72.
  2. Wigger M, Wutzke KD: Whole-body protein turnover in nephrotic subjects: a comparison between different degrees of proteinuria. Isotopes Environ Health Stud 2004;40:155-61.
  3. Wutzke KD, Lorenz H: The effect of L-carnitine on fat oxidation, protein turnover and body compostion in slightly obese subjects. Metabolism 2004;53:1002-6.
  4. Wutzke KD, Oetjens I: 13C- and 15N-incorporation of doubly stable isotope labelled Lactobacillus johnsonii in humans. Eur J Clin Nutr 2005;59:1167-72.
  5. Wutzke KD, Sattinger V: 15N-excretion of heat-killed Lactobacillus casei in humans. Eur J Clin Nutr 2006;60:847-52.
  6. Wutzke KD, Schütt M: The duration of enzyme induction in orocaecal transit time measurements. Eur J Clin Nutr 2007;61:1162-6.
  7. Schönhusen U, Kuhla S, Zitnan R, Wutzke KD, Huber K, Moors S, Voigt J: Effect of a soy protein-based diet on ribonucleic acid metabolism in the small intestinal mucosa of goat kids. J Dairy Sci 2007;90:2404-12.
  8. Götz KP, Staroske N, Radchuk R, Emery RJ, Wutzke KD, Herzog H, Weber H: Uptake and allocation of carbon and nitrogen in Vicia narbonensis plants with increased seed sink strength achieved by seed-specific expression of an amino acid permease. J Exp Bot 2007;58:3183-95.
  9. Wutzke KD, Forberger A, Wigger M: Effect of alcohol consumption on the liver detoxification capacity as measured by [13C]methacetin- and [methyl-13C]methionine-breath tests. Isotopes Environ Health Stud 2008;44:219-26.
  10. Wutzke KD, Berg D, Haffner D: The metabolic fate of doubly stable isotope labelled heat-killed Lactobacillus johnsonii in humans. Eur J Clin Nutr 2008;62:197-202.
  11. Wutzke KD, Wigger M: Effect of alcohol consumption on the liver detoxication capacity as measured by [13C2]aminopyrine- and L-[1-13C]phenylalanine-breath tests. Isotopes Environ Health Stud 2009;45:185-91.
  12. Wutzke KD, Mix J: The metabolic fate of doubly labelled lactose-[13C, 15N]ureide after pre-dosing with different ureides. Eur J Clin Nutr 2010;64:733-8.
  13. Wutzke KD, Lotz M, Zipprich C: The effect of pre- and probiotics on the colonic ammonia metabolism in man as measured by lactose-[15N2]ureide. Eur J Clin Nutr 2010;64:1215-21.
  14. Schellekens RCA, Olsder G, Maurer M, Kosterink J, Woerdenbach HJ, Wutzke KD, Frijlink HW, Stellaard F: 13C-urea and 15N2-urea: elegant markers to define location and kinetics of in vivo release from a colon-targeted delivery device in men. Eur J Clin Invest 2010;40 [Suppl 1]:66.
  15. Wutzke KD, Krentz H, Bruns G: Effect of alcohol consumption on whole-body protein turnover in healthy adults. Isot Environ Health Stud 2011;47:26-33.
  16. Wutzke KD: The development and application of new 15N-tracer substances for measuring the whole-body protein turnover rates in the human, especially in neonates: a review. Isot Environ Health Stud 2012;48:239-58.
  17. Maurer MJ, Schellekens RC, Wutzke KD, Dijkstra G, Woerdenbag HJ, Frijlink HW, Kosterink JG, Stellaard F: A non-invasive, low-cost study design to determine the release profile of colon drug delivery systems: a feasibility study. Pharm Res 2012;29:2070-8.
  18. Wutzke KD, Scholübbers D: The metabolic effect of resistant starch and yoghurt on the renal and faecal nitrogen and ammonia excretion in humans as measured by lactose-[15N2]ureide. Isot Environ Health Stud 2013;49:464-70.
  19. Maurer MJM, Schellekens RCA, Wutzke KD, Stellaard F: Isotope labelled urea to test colon drug delivery devices in vivo: principles, calculations and interpretations. Isot Environ Health Stud 2013;49:473-91.
  20. Maurer JM, Schellekens RC, van Rieke HM, Stellaard F, Wutzke KD, Buurman DJ, Dijkstra G, Woerdenbag HJ, Frijlink HW, Kosterink JG: ColoPulse tablets perform comparably in healthy volunteers and Crohn's patients and show no influence of food and time of food intake on bioavailability. J Control Release 2013;172:618-24.
  21. Maurer JM, Schellekens RCA, van Rieke HM, Wanke C, Iordanov V, Stellaard F, Wutzke KD, Dijkstra G, van der Zee M, Woerdenbag HJ, Frijlink HW, Kosterink JGW: Gastro-intestinal pH and transit time profiling in healthy volunteers using the IntelliCap system confirms ileo-colonic release of ColoPulse tablets. PLoS One 2015 Jul 15;10(7):e0129076.
  22. Wutzke KD, Tisztl M, Salewski B, Glass A: Dietary fibre- rich resistant starches promote ammonia detoxification in the human colon as measured by lactose- [15N2] ureide. Isot Environ Health Stud 2015;51:488-96.

Impactpoints 2004-2016: 27.95

Patents

  1. Plath C, Russow R, Sich I, v Bismarck P, Wutzke KD: Verfahren zur Bestimmung der [15N]Stickstoffmonoxid-Exhalation zur Diagnostik und Therapie bei Erkrankungen des Menschens. DE 197 19 098 C1;1999, IPC: A61K 49/00

Dissertations and third-party funds of the Research Laboratory

  1. Björn Glasenapp: Utilisation of glycosyl ureides for measuring oro-caecal transit time (OCTT). (Scientific Award of the GPGE, Potsdam 2002) Defense of doctoral theses 2002: magna cum laude
  2. Frederick Palm: The impact of L-carnitine on fat oxidation. Defense of doctoral theses 2003: cum laude (in cooperation with Hero Ltd. Lenzburg, Switzerland)
  3. Daniela Gohr: The effect of lactose ureide dosage on OCTT measurement. Defense of doctoral theses 2003: cum laude
  4. Kirstin Händler: The nutritional effect on gastric emptying and OCTT. Defense of doctoral theses 2004: cum laude
  5. Iris Oetjens: Utilisation of doubly labelled probiotic Lactobacillus acidophilus (LC1). (Grant of the Karl and Veronica Carstens-Foundation) Defense of doctoral theses 2004: magna cum laude
  6. Manja Schütt: The duration of enzyme induction by lactose ureide on OCTT measurement. Defense of doctoral theses 2005: cum laude
  7. Henrik Lorenz: The impact of L-carnitine on fat oxidation and protein turnover in slightly obese subjects. Defense of doctoral theses 2005: magna cum laude (in cooperation with Lonza Ltd., Basle)
  8. Verena Sattinger: Utilisation of killed 15N-labelled L. casei. (Grant of the East Tennessee State University) Defense of doctoral theses 2005: magna cum laude
  9. Dominika Berg: Utilisation of killed, doubly labelled LC1. Defense of doctoral theses 2006: cum laude
  10. Anke Meinke: The impact of alcohol on detoxication capacity of the liver measured by [13C]methacetin- and [methyl-13C]methionine-breath tests. Defense of doctoral theses 2006: magna cum laude
  11. Susanne Müller (Prof. Wigger): The effect of L-carnitine on fat oxidation in nephrotic syndrome. Defense of doctoral theses 2006: cum laude
  12. Christian Gereke: The impact of L-carnitine on glucose oxidation. Defense of doctoral theses 2007: cum laude (in cooperation with Lonza Ltd., Basle)
  13. Juliane Haase (Prof. Plath): The influence of complementary food on gastric emptying and OCTT in infants. Defense of doctoral theses 2008: cum laude (in cooperation with HiPP, Pfaffenhofen)
  14. Martin Dürr: The effect of L-carnitine dosage on gastric emptying and OCTT. Defense of doctoral theses 2008: cum laude
  15. Carolin Griesheim: The effect of L-carnitine on fat oxidation in elderly subjects. Defense of doctoral theses 2008: cum laude (in cooperation with Lonza Ltd., Basle)
  16. Tina Teichmann (Prof. Wigger): The effect of cyclosporine A on fat oxidation of transplanted subjects. Defense of doctoral theses 2008: cum laude
  17. Susanne Staar: ...[methyl-13C]ethanol and [1-13C]galactose-breath tests. Defense of doctoral theses 2009: magna cum laude
  18. Ramona Dummer: The effect of growth hormon therapy on fat oxidation. Defese of the doctoral theses 2009: cum laude (in cooperation with Pharmacia, Erlangen)
  19. Rebekka Wichmann: Excretion and incorporation of orally administered 15N-labelled yeast nucleic acids in healthy adults. Defese of the doctoral theses 2009: cum laude
  20. Johanna Mix: The metabolic fate of doubly labelled lactose-[13C, 15N]ureide after pre-dosing with different ureides. Defense of the doctoral theses 2011: summa cum laude (in cooperation with Campro Scientific, Berlin)
  21. Andrea Popp: The effect of L-carnitine supplementation on fat oxidation, respiratory quotient, and protein turnover in adipose subjects. Defense of the doctoral theses 2011: cum laude (in cooperation with Lonza Ltd., Basle)
  22. Katharina Roost: Effect of resistant starch on fat oxidation in humans. Defense of the doctoral theses 2012: cum laude
  23. Antje Krzyzanowski (PD Dr. Walther): OCTT measurements in healthy children and in children suffering from chronic inflammatory bowel diseases. Defense of the doctoral theses 2013: cum laude
  24. Gerrit Bruns: The impact of alcohol consumption on protein turnover in men measured by using 15N-labelled yeast protein. Defense of the doctoral theses 2013: cum laude
  25. Carina Zipprich: Lactose-[15N2]ureide: a biomarker for assessing pro- and prebiotic effects in humans. Defense of the doctoral theses 2014: cum laude (in cooperation with Emsland Group GmbH, Emlichheim)
  26. Kerstin Schmidek: The effect of different resistant starches on fat oxidation and respiratory quotient in humans. Defense of the doctoral theses 2016: cum laude
  27. Silvia Nern: The impact of cigarette smoking and caffeine on detoxication capacity of the liver measured by [13C2]aminopyrine and [13C3]caffeine-breath tests. (Work submitted)
  28. Debora Scholübbers: The synbiotic effect of pre- and probiotics on the colonic ammonia metabolism in humans as measured by lactose-[15N2]ureide.
  29. Anne Kathrin Neururer: ...[1-13C]ketoisocaproic acid and [methyl-13C3]caffeine-breath tests.
  30. Bernhard Zimmermann: The impact of L-carnitine on fat oxidation, respiratory quotient, and protein turnover in adipose subjects. (FORUN-project, in cooperation with Lonza Ltd., Basle)
  31. Steffen Büchner: The effect of an L-carnitine/ omega-3 fatty acid mixture on fat oxidation in obese subjects. (in cooperation with STADA, Bad Vibel)
  32. Martin Lange: ...[methyl 13C2]aminopyrine- and [1-13C]phenylalanine-breath tests.
  33. N.n.: The impact of alcohol consumption on gastric emptying and OCTT in men.
  34. Tabea Stolz (PD Dr. Walther): 13CO2- and H2 breath tests for analysing of the bacterial overgrowth syndrome in childhood.
  35. N.n.: Utilisation of 15N-labelled L. casei.
  36. Michael Tisztl: The effect of different resistant starches on the colonic ammonia metabolism in humans as measured by lactose-[15N2]ureide. (in cooperation with Campro Scientific, Berlin)
  37. Prof. Jörg Dötsch: Randomized, multicenter cross-over study investigating the effect of bicarbonate-based solutions (Physioneal 35 vs. 40) on protein metabolism in children and adolescents on chronic peritoneal dialysis. EudraCT number: 2005-005434-12. (in cooperation with the University of Erlangen, Prof. Rascher)
  38. N.n.: Effect of resistant starch on fat oxidation, body composition, and respiratory quotient in humans.
  39. Patricia Winkler (PD Dr. Walther): H2-breath tests for analysing the bacterial overgrowth in children suffering from irritable bowel syndrome.
  40. Anne-Sophie Tröndle: The effect of resistant starch on the colonic ammonia metabolism in humans as measured by lactose-[15N2]ureide after pre-dosing. (in cooperation with Campro Scientific, Berlin)
  41. Sarah Michalski: The effect of resistant starch on the colonic ammonia metabolism, the orocaecal transit time, the hydrogen, and the methan generation, respectively, as measured by lactose-[13C,15N]ureide. (in cooperation with Campro Scientific, Berlin)
  42. Alexander Gröner: Effect of different resistant starchs on fat oxidation, body composition, and respiratory quotient in humans.
  43. Patricia Frank: The effect of corn starch, lactulose and L. casei on the colonic ammonia metabolism, the orocaecal transit time, the hydrogen, and the methan generation, respectively, as measured by lactose-[13C,15N]ureide. (in cooperation with Campro Scientific, Berlin)

Net receipts (1999-2016): 260,000 €

Own laboratory syntheses

1. lactose-[13C,15N]ureide

2. 15N-labelled yeast protein

3. 15N-labelled yeast nucleic acids

Equipment of the Research Laboratory

 

 

Isotope ratio mass spectrometer
(Tracer Mass 20-20, ANCA-GSL, and High Temp EA, SerCon, U.K. )
for measuring the isotopes of 13C, 15N, and 18O from CO2 or solid samples
Non dispersive infrared spectrometer
(FANci2, FAN Leipzig)
for measuring the isotope 13C from CO2
Fermenter
(Biostad B, B. Braun Melsungen)
for yeast labelling with stable isotopes
Ultrasonic generator
(Labsonic U, B. Braun)
for destroying yeast cells
Methan and hydrogen analyser
(BreathTracker, Quintron, Milwaukee, WI, USA)
for measuring methan, hydrogen and CO2
Hydrogen-analyser
(Stimotron, Wendelstein)
for measuring the H2-concentration in breath
Indirect calorimeter
(Oxycon Alpha, E. Jaeger, Würzburg)
for measuring the respiratory quotient